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Application of high-resolution melting-curve analysis on pvpA gene for detection and classification of Mycoplasma

Shabnam Hashemi1, Mohammadreza Mahzounieh1, Nariman Sheikhi2

  • 1Research Institute for Zoonotic Diseases and Department of Pathobiology, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

Microbial Pathogenesis
|July 1, 2018
PubMed

Insights

Genomic diversity of Mycoplasma gallisepticum (MG) in Iran was analyzed using pvpA gene sequencing and HRM. Findings suggest endemic chicken and partridge strains, with a distinct peacock strain posing a potential poultry industry threat.

Area of Science:

  • Avian Pathogen Genomics
  • Molecular Epidemiology
  • Poultry Health

Background:

  • Mycoplasma gallisepticum (MG) causes significant economic losses in the poultry industry.
  • Understanding MG genomic diversity is crucial for disease control and prevention.
  • Previous studies have not fully characterized Iranian MG field strains.

Purpose of the Study:

  • To determine the genomic diversity of 14 Mycoplasma gallisepticum field strains from Iran.
  • To differentiate between field and vaccine strains using High-Resolution Melting (HRM) analysis.
  • To investigate the evolutionary relationships and potential origins of Iranian MG strains.

Main Methods:

  • Polymerase chain reaction (PCR) and partial sequencing of the pvpA gene.
  • Development and application of High-Resolution Melting (HRM) technique.
  • Phylogenetic analysis of pvpA gene sequences.

Main Results:

  • Sequencing revealed nucleotide deletions and substitutions in the pvpA gene among different avian hosts.
  • Phylogenetic analysis clustered Iranian MG strains into two distinct groups.
  • HRM analysis successfully differentiated field strains from the vaccine strain, correlating with sequencing data.

Conclusions:

  • Chicken and partridge MG strains likely share a common, endemic source in Iran.
  • A distinct peacock MG strain, potentially exotic, may pose a threat to poultry.
  • The HRM technique is a rapid and affordable method for revealing molecular variations in MG pvpA genes.
Keywords:
HRMIranMycoplasma gallisepticumpvpA gene

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