Evaluation of Various Sterilization Processes of Orthodontic Instruments using Biological Indicators and Conventional...

This study aimed to compare the efficiency of various sterilization procedures using conventional spore monitoring method, i.e., by using swab test and biological indicators and to determine the efficiency of cold sterilization by using Bioclenz-G (2% glutaraldehyde) solution.

Each group was divided into medium load (containing 15 sets of instruments) and heavy load (containing 30 sets of instruments). Each group was tested 15 times for medium and heavy loads. Two groups are swab tested control group and experimental group with three different methods of sterilization: hot air oven, cold sterilization, and ethylene dioxide sterilization.

Spores were present in all the groups tested for 10 minutes cycle, in comparison with no spore growth in any of the groups tested for a 10-hour cycle.

All methods of sterilization showed complete sterilization of instruments when monitored with biological indicators. One group of heavy load in steam autoclave and one group each of medium load and heavy load in hot air oven sterilizer showed sterilization failure when monitored with the conventional swab test method.

This study proves the efficacy and durability of various sterilization procedures.