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Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
Published on: October 6, 2022
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A novel sample holder for 4D live cell imaging to study cellular dynamics in complex 3D tissue cultures
D Septiadi1, J Bourquin2, E Durantie2
1Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700, Fribourg, Switzerland. dedy.septiadi@unifr.ch.
Scientific Reports
|July 1, 2018
Summary
This study introduces 4D live cell imaging, a novel technique for visualizing 3D co-cultures and cell dynamics over 24 hours. This method enables detailed monitoring of particle uptake and translocation across tissue membranes.
Area of Science:
- Biomedical Engineering
- Cell Biology
- Tissue Engineering
Background:
- Three-dimensional (3D) co-cultures are crucial for mimicking cellular dynamics in tissue engineering.
- Advanced imaging techniques are needed to observe cellular processes in real-time within these complex environments.
Purpose of the Study:
- To develop and present a novel 4D live cell imaging technique for enhanced visualization of 3D co-cultures.
- To enable direct, long-term (up to 24 hours) observation of cellular behavior and interactions.
Main Methods:
- Integration of a novel sample holder with time-lapse fluorescence imaging.
- Implementation of a 4D live cell imaging approach for dynamic cell visualization.
- Application of the technique to study particle uptake and translocation kinetics.
Main Results:
- Successful direct visualization of various cells within 3D co-cultures for up to 24 hours.
- Demonstrated capability to monitor the kinetics and dynamics of particle uptake by cells.
- Validated the technique for observing particle translocation across engineered tissue membranes.
Conclusions:
- The developed 4D live cell imaging technique significantly advances the study of cellular dynamics in 3D co-cultures.
- This approach provides unprecedented insights into cellular uptake and transport mechanisms relevant to tissue engineering and drug delivery.
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