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Related Concept Videos

Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Compared with pure water, the solubility of an ionic compound is less in aqueous solutions containing a common ion (one also produced by dissolution of the ionic compound). This is an example of a phenomenon known as the common ion effect, which is a consequence of the law of mass action that may be explained using Le Chȃtelier’s principle. Consider the dissolution of silver iodide:
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Transcription Elongation Factors02:35

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Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
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Factors Affecting Drug Distribution: Miscellaneous Factors01:19

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Electrolytes: van't Hoff Factor03:08

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Colligative Properties of Electrolytes
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Factors Affecting Protein-Drug Binding: Drug-Related Factors01:18

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Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
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Related Experiment Video

Updated: Feb 8, 2026

GST-His purification: A Two-step Affinity Purification Protocol Yielding Full-length Purified Proteins
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Purification of Microprocessor-Associated Factors.

Ross A Cordiner1, Sara Macias2

  • 1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Alle 3, Bldg. 1130, 8000 Aarhus C, Aarhus, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|July 1, 2018
PubMed
Summary

Researchers developed a method to purify the Microprocessor complex, revealing new factors involved in microRNA (miRNA) biogenesis and RNA turnover. This technique aids in identifying unknown protein associations.

Keywords:
DGCR8DroshaImmunoprecipitationMass spectrometryPri-miRNA processingRNA processingWestern blotmiRNAs

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • The Microprocessor complex, comprising DGCR8 and Drosha, is crucial for initiating microRNA (miRNA) biogenesis in mammalian cell nuclei.
  • Associated co-factors can modulate the efficiency of this complex's RNA binding and cleavage activities.

Purpose of the Study:

  • To develop a straightforward method for purifying the DGCR8-Drosha complex.
  • To enable the identification of novel associated factors using mass spectrometry or western blot analysis.
  • To investigate the roles of these factors in miRNA biogenesis and RNA metabolism.

Main Methods:

  • Purification of the DGCR8-Drosha complex.
  • Coupling purification with mass spectrometry for protein identification.
  • Coupling purification with western blot for factor validation.

Main Results:

  • A robust method for DGCR8 and Drosha complex purification was established.
  • The purification strategy successfully identified associated factors.
  • A novel DGCR8-dependent, Drosha-independent complex implicated in RNA turnover was discovered.

Conclusions:

  • The developed purification method is effective for identifying novel protein interactions within the Microprocessor complex.
  • This approach has uncovered a new complex involved in RNA turnover, expanding our understanding of RNA metabolism.
  • Further research into this novel complex may reveal new regulatory pathways in gene expression.