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Related Concept Videos

Cell Culture01:21

Cell Culture

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Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
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Stem Cell Culture01:17

Stem Cell Culture

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Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...
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Epithelial Tissues and Their Functions01:23

Epithelial Tissues and Their Functions

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Epithelial tissues are large sheets of cells covering all of the surfaces of the body. These surfaces can be internal or external, for example, skin, airways, the digestive tract, the urinary system, and the reproductive system. Hollow organs and body cavities that do not connect to the body's exterior, including blood vessels and serous membranes, are lined by epithelial tissue known as the endothelium.
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Classification of Epithelial Tissues: Overview01:22

Classification of Epithelial Tissues: Overview

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Epithelial tissues are classified according to the shape of the cells and the number of cell layers formed. Cell shapes can be squamous (flattened and thin), cuboidal (square-like, as wide as it is tall), or columnar (rectangular, taller than it is wide). Additionally, the nucleus shape helps identify the type of epithelial cells. Squamous cells have flattened disc-shaped nuclei, cuboidal cells have spherical nuclei, and columnar cells have elongated nuclei.
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Classification of Epithelial Tissues: Stratified Epithelium01:29

Classification of Epithelial Tissues: Stratified Epithelium

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Stratified epithelium consists of several stacked layers of cells. They provide the durability to withstand constant physical and chemical attacks. Stratified epithelium is named after the shape of the most apical layer of cells. Stratified squamous epithelium is the most common type found in the human body. In this tissue, the apical cells are squamous, whereas the basal layer contains either columnar or cuboidal cells. The basal cells divide to form new daughter cells, which gradually become...
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Classification of Epithelial Tissues: Glandular Epithelium01:20

Classification of Epithelial Tissues: Glandular Epithelium

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The glandular epithelium is made of one or more epithelial cells modified to synthesize and secrete chemical substances. Glandular epithelia can be classified based on cell number. Unicellular glands have individual secretory cells scattered across the epithelial monolayer. In contrast, multicellular glands consist of a hollow tubular duct attached to the cluster of secretory cells located in the deep pockets.
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Related Experiment Video

Updated: Feb 8, 2026

In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane
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In Vitro Culture of Epithelial Cells from Different Anatomical Regions of the Human Amniotic Membrane

Published on: November 28, 2019

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Amniotic Epithelial Cell Culture.

Angelo Canciello1, Luana Greco2, Valentina Russo2

  • 1Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. acanciello@unite.it.

Methods in Molecular Biology (Clifton, N.J.)
|July 1, 2018
PubMed
Summary
This summary is machine-generated.

Ovine amniotic epithelial cells (oAEC) show promise for regenerative medicine. This study presents an improved protocol to maintain their native phenotype during in vitro culture, enhancing therapeutic potential.

Keywords:
Amniotic epithelial cellsCell cultureEpithelial-mesenchymal transitionIn vitro amplificationProgesteroneStem cells

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Area of Science:

  • Stem cell research
  • Regenerative medicine
  • Animal models

Background:

  • Ovine amniotic epithelial cells (oAEC) possess significant regenerative and immunomodulatory properties.
  • Sheep serve as a valuable translational animal model for pre-clinical studies in regenerative medicine.
  • Standardization of cell culture is crucial for maintaining oAEC phenotype and therapeutic efficacy.

Purpose of the Study:

  • To describe an improved protocol for culturing ovine amniotic epithelial cells (oAEC).
  • To ensure the preservation of the native epithelial phenotype of oAEC during in vitro amplification.
  • To enhance the in vivo therapeutic potential and clinical outcomes of oAEC.

Main Methods:

  • Development and application of a novel cell culture protocol for oAEC.
  • In vitro amplification of oAEC using the improved protocol.
  • Phenotypic analysis of oAEC post-culture to assess preservation.

Main Results:

  • The improved protocol successfully preserves the native epithelial phenotype of oAEC after in vitro amplification.
  • Demonstrated ability to maintain key cellular characteristics essential for therapeutic applications.
  • Indicated enhanced potential for pre-clinical studies and clinical translation.

Conclusions:

  • The developed protocol is effective in maintaining the native phenotype of ovine amniotic epithelial cells.
  • This standardization is critical for maximizing the therapeutic efficacy of oAEC in regenerative medicine.
  • The findings support the use of oAEC in pre-clinical research and potential clinical applications.