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Author Spotlight: Enhanced Method for Isolating Pure Zebrafish Stage I Oocytes
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Bioencapsulation of Oocytes and Granulosa Cells
Massimo Faustini1, Giulio Curone2, Maria L Torre3
1Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy.
Methods in Molecular Biology (Clifton, N.J.)
|July 1, 2018
Abstract:
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.
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