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Updated: Feb 8, 2026

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products
Published on: October 4, 2019
Diterpenoids and lignans from the leaves of Tripterygium wilfordii
Fangyou Chen1, Chuangjun Li1, Jie Ma1
1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, People's Republic of China.
Abstract:
Three new diterpenoids (1-3) and three new esterifying lignans (4-6) were isolated from the leaves of Tripterygium wilfordii. The structures of these compounds were elucidated on the basis of spectroscopic techniques (UV, IR, NMR, HRESIMS, and ECD method). At 10 μmol/L, compounds 4-6 showed moderate inhibitory effects on nitric oxide production in LPS-induced macrophages with inhibitory rate >80%.
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In general, in a nucleophilic substitution reaction, a nucleophile displaces a functional group, called the leaving group, from the substrate to give a substituted product. A leaving group departs the substrate molecule through heterolytic cleavage, taking the pair of electrons with it to become a relatively stable weak base in the form of an anion or a neutral molecule.
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