Responses of soil microbial community structure changes and activities to biochar addition: A meta-analysis

The objective of this study was to investigate responses of soil microbial community structure changes and activities to biochar addition under different biochar characteristics, soil properties, and experiment conditions. A meta-analysis was conducted based on 265 datasets from 49 published studies. Results showed that biochar addition significantly increased the ratios of soil fungi to bacteria (F/B) and the ratios of Gram-positive bacteria to Gram-negative bacteria (G+/G-), and microbial biomass and activities. The enhancement of F/B ratios was most significant with addition of biochars produced at low temperatures to soils with lower pH and nutrients in a long-term condition, which improved ecosystem stability of agricultural soils. The F/B ratios were mainly affected by biochar nutrients, soil nutrients, and soil pH values. Biochar nutrients and structural properties (i.e., surface area and porosity) also played the important role in enhancing G+/G-, total microbial biomass, and activities of bacteria, fungi, and actinomycetes. The G+/G- ratios increased the most with addition of biochars produced with medium temperatures and residue accompanied with fertilizers in dry land (dried farmland) soils. High biochar load greatly improved the total phospholipid fatty acids, and activities of bacteria, fungi, and actinomycetes in fine/coarse, paddy soils, and soils with low nutrients, in turn increased the soil nutrient cycling. In addition, the structural properties of biochars were the most influencing factor to increase total microbial biomass and actinomycete activity. Overall, the enhancement of microbial activities and community structure shifts under biochar addition should promote soil nutrients cycling and carbon sequestration, and improve crop yields.