Colorimetric chiral recognition of D/L-phenylalanine based on triangular silver nanoplates

A new colorimetric analysis approach for chiral recognition of D- and L-forms of phenylalanine (phe) was developed based on triangular silver nanoplates (TAg-NPs). The TAg-NPs could be used as chiral colorimetric probes for D- and L-forms of phe. Upon addition of D-phe to TAg-NPs solution, a color change from blue to purple to pink could be observed, while no obvious color change was found on addition of L-phe. L-phe could prevent the TAg-NPs from being etched to small size particles while the protective effect of D-phe was weak. Moreover, the enantiomeric excess of D-phe could be determined using the proposed chiral assay in the percentage of L-phe from 0 to 100% with a correlation coefficient of 0.9855. The phenomenon could be monitored by bare eyes and quantified analysis by UV-Vis spectrophotometry. The developed approach had several advantages, such as simplicity, visualization, short analysis time and low cost. This study presented a fast visualization analysis method of chiral D/L-phenylalanine and may lay the foundation for the development of visualization chiral recognition of other target analytes.