Characterising skeletal muscle haemoglobin saturation during exercise using near-infrared spectroscopy in chronic...

Chronic kidney disease (CKD) patients have reduced exercise capacity. Possible contributing factors may include impaired muscle O₂ utilisation through reduced mitochondria number and/or function slowing the restoration of muscle ATP concentrations via oxidative phosphorylation. Using near-infrared spectroscopy (NIRS), we explored changes in skeletal muscle haemoglobin/myoglobin O₂ saturation (SMO₂%) during exercise.

24 CKD patients [58.3 (± 16.5) years, eGFR 56.4 (± 22.3) ml/min/1.73 m²] completed the incremental shuttle walk test (ISWT) as a marker of exercise capacity. Using NIRS, SMO₂% was measured continuously before, during, and after (recovery) exercise. Exploratory differences were investigated between exercise capacity tertiles in CKD, and compared with six healthy controls.

We identified two discrete phases; a decline in SMO₂% during incremental exercise, followed by rapid increase upon cessation (recovery). Compared to patients with low exercise capacity [distance walked during ISWT, 269.0 (± 35.9) m], patients with a higher exercise capacity [727.1 (± 38.1) m] took 45% longer to reach their minimum SMO₂% (P = .038) and recovered (half-time recovery) 79% faster (P = .046). Compared to controls, CKD patients took significantly 56% longer to recover (i.e., restore SMO₂% to baseline, full recovery) (P = .014).

Using NIRS, we have determined for the first time in CKD, that favourable SMO₂% kinetics (slower deoxygenation rate, quicker recovery) are associated with greater exercise capacity. These dysfunctional kinetics may indicate reduced mitochondria capacity to perform oxidative phosphorylation-a process essential for carrying out even simple activities of daily living. Accordingly, NIRS may provide a simple, low cost, and non-invasive means to evaluate muscle O₂ kinetics in CKD.