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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions
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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

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Dysregulated circRNAs in plasma from active tuberculosis patients

Zhengjun Yi1, Kunshan Gao1, Ruifang Li2

  • 1Department of Laboratory Medicine, Key Laboratory of Clinical Laboratory Diagnostics in Universities of Shandong, Weifang Medical University, Weifang, China.

Journal of Cellular and Molecular Medicine
|July 2, 2018
PubMed

Insights

Plasma circular RNAs (circRNAs) show altered expression in active tuberculosis (TB). Hsa_circRNA_103571 may serve as a diagnostic biomarker for active TB, offering insights into disease mechanisms.

Area of Science:

  • Molecular Biology
  • Genomics
  • Immunology

Background:

  • Endogenous circular RNAs (circRNAs) are implicated in various diseases.
  • The role of circRNAs in active tuberculosis (TB) pathogenesis is not yet understood.

Purpose of the Study:

  • To investigate the plasma circRNA expression profile in active TB.
  • To identify potential circRNA biomarkers for active TB diagnosis.
  • To elucidate the role of circRNAs in active TB pathogenesis.

Main Methods:

  • CircRNA expression profiling using circRNA microarrays in active TB plasma.
  • Validation of dysregulated circRNAs via quantitative real-time PCR (qRT-PCR).
  • Bioinformatic analysis for circRNA-miRNA-mRNA interactions, gene ontology (GO), and KEGG pathway analysis.
  • Receiver operating characteristic (ROC) curve analysis for diagnostic value assessment.

Main Results:

  • A total of 75 circRNAs were significantly dysregulated in active TB plasma.
  • Hsa_circRNA_103571 showed a significant decrease in active TB patients.
  • Hsa_circRNA_103571 interacts with TB-related microRNAs (miRNAs) like miR-29a and miR-16.
  • Bioinformatics analysis linked hsa_circRNA_103571 to pathways including ras signaling and T-/B-cell receptor signaling.
  • ROC analysis indicated hsa_circRNA_103571 has significant diagnostic value for active TB.

Conclusions:

  • Circulating circRNA dysregulation is associated with active TB pathogenesis.
  • Hsa_circRNA_103571 demonstrates potential as a diagnostic biomarker for active TB.
  • circRNA-miRNA-mRNA interactions may reveal novel mechanisms underlying active TB.
Keywords:
biomarkercircRNAmiRNApathogenesistuberculosis

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