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Updated: Feb 8, 2026
09:08
Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
Published on: December 22, 2020
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Multisensor Imaging-From Sample Preparation to Integrated Multimodal Interpretation of LA-ICPMS and MALDI MS Imaging
Matthias Holzlechner1, Maximilian Bonta1, Hans Lohninger1
1Institute of Chemical Technologies and Analytics , TU Wien , Getreidemarkt 9 , 1060 Vienna , Austria.
Analytical Chemistry
|July 3, 2018
Summary
This study combined laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for chemical imaging. The techniques revealed platinum and lipid distributions in a mesothelioma tissue sample, correlating their presence.
Area of Science:
- Life Sciences
- Biochemistry
- Analytical Chemistry
Background:
- Chemical imaging techniques are crucial for understanding biochemical processes in biological samples.
- Multisensor imaging, combining multiple analysis techniques, offers deeper insights than single methods.
- Advances in lateral resolution and speed are enhancing chemical imaging capabilities.
Purpose of the Study:
- To demonstrate the benefit of combining LA-ICPMS and MALDI MS for chemical imaging of a human malignant pleural mesothelioma sample.
- To correlate platinum distribution with lipid profiles in a cisplatin-treated patient sample.
- To showcase the value of advanced chemical imaging in Life Sciences research.
Main Methods:
- Human malignant pleural mesothelioma tissue sample analyzed using LA-ICPMS and MALDI MS.
- Simultaneous chemical imaging with a lateral resolution of 40 μm.
- Tandem MS experiments for lipid class verification and advanced statistical analysis for data correlation.
Main Results:
- LA-ICPMS provided quantitative platinum and elemental distribution.
- MALDI MS revealed comprehensive lipid distributions (positive and negative polarity).
- Correlations were identified between platinum, phosphorus, and specific lipid distributions.
Conclusions:
- Combining LA-ICPMS and MALDI MS offers superior information compared to single-modality imaging.
- Advanced chemical imaging provides detailed insights into biochemical processes and drug distribution in tissues.
- This proof-of-principle study highlights the potential of integrated imaging for Life Sciences.
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