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Bidirectional SV40 transcription mediated by tandem Sp1 binding interactions
Science (New York, N.Y.)
|November 1, 1985
Summary
The SV40 promoter
Area of Science:
- Molecular Biology
- Genetics
- Biochemistry
Background:
- The SV40 promoter contains 21-base pair repeat elements with six GC-boxes.
- Each GC-box binds the cellular transcription factor Sp1 with varying affinity.
Purpose of the Study:
- To investigate the role of Sp1 binding to specific GC-boxes in SV40 promoter activity.
- To differentiate the binding sites of Sp1 for early and late RNA synthesis.
Main Methods:
- In vitro transcription assays.
- Analysis of Sp1 binding to SV40 promoter GC-boxes.
Main Results:
- Sp1 interaction with GC-boxes I, II, and III mediates in vitro SV40 early RNA synthesis.
- Sp1 binding to GC-boxes III, V, and VI mediates SV40 late RNA synthesis.
Conclusions:
- Specific GC-boxes within the SV40 promoter are differentially utilized by Sp1 for early and late transcription.
- This differential binding mechanism regulates the directionality of SV40 RNA synthesis.
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