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Development of Compendium for Esophageal Squamous Cell Carcinoma
03:36

Development of Compendium for Esophageal Squamous Cell Carcinoma

Published on: April 12, 2024

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miR-135 promotes proliferation and stemness of oesophageal squamous cell carcinoma by targeting RERG

Yan Zhang1, Shuang Ren2, Fengfeng Yuan1

  • 1a Department of Thoracic Surgery , The First Affiliated Hospital of Zhengzhou University, Zhengzhou University , Zhengzhou , China.

Artificial Cells, Nanomedicine, and Biotechnology
|July 3, 2018
PubMed

Insights

MicroRNA-135 (miR-135) promotes esophageal squamous cell carcinoma (ESCC) development and progression. Upregulated miR-135 in ESCC indicates poor prognosis and can be a potential diagnostic biomarker.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial regulators of tumorigenesis, influencing cell cycle, mobility, and senescence.
  • miRNAs' resistance to degradation makes them valuable for cancer diagnosis and prognosis.
  • Esophageal squamous cell carcinoma (ESCC) requires identification of specific biomarkers for effective management.

Purpose of the Study:

  • To investigate microRNA expression profiles in ESCC.
  • To identify specific microRNAs that can serve as diagnostic or prognostic biomarkers for ESCC.
  • To elucidate the role of identified microRNAs in ESCC progression and identify their molecular targets.

Main Methods:

  • miRNA profiling was performed to identify differentially expressed miRNAs in ESCC.
  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to validate miRNA expression levels.
  • Cox regression and survival analyses were conducted to correlate miRNA expression with clinical parameters.
  • In vitro assays assessed the functional impact of miRNA modulation on cancer cell behavior.
  • Bioinformatic tools were employed for target prediction and functional enrichment analysis.

Main Results:

  • miR-135, miR-451, and miR-186 were identified as significantly dysregulated in ESCC.
  • miR-135 was consistently upregulated in ESCC cell lines (TE2, TE9) and correlated with advanced tumor stages.
  • Higher miR-135 expression was observed in metastatic samples, and its downregulation inhibited ESCC cell proliferation and invasion.
  • RERG was identified as a direct target of miR-135, and its downregulation suppressed cell proliferation and sphere formation.

Conclusions:

  • miR-135 plays a significant role in promoting ESCC development and progression.
  • miR-135 demonstrates potential as a diagnostic and prognostic biomarker for ESCC.
  • Targeting miR-135 or its downstream effectors like RERG may offer therapeutic strategies for ESCC.
Keywords:
GTPase signallingOesophageal squamous cell carcinomaRAS-like oestrogen-regulated growth inhibitormicroRNA

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