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Absent expression of miR-30a promotes the growth of lung cancer cells by targeting MEF2D

Nianxu Luan1, Yi Wang1, Xuedong Liu1

  • 1Department of Pneumology, Qingdao Municipal Hospital, Qingdao, Shandong 266011, P.R. China.

Oncology Letters
|July 3, 2018
PubMed

Insights

MicroRNA (miR)-30a suppresses lung cancer growth by targeting MEF2D. This study reveals miR-30a

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Aberrant microRNA (miR) expression is implicated in various cancers.
  • The role of the miR-30 family in lung cancer pathogenesis requires further investigation.
  • Myocyte enhancer factor 2D (MEF2D) is an oncogene implicated in liver and lung cancers.

Purpose of the Study:

  • To elucidate the role of miR-30a in lung cancer.
  • To investigate the relationship between miR-30a and MEF2D in lung cancer.
  • To determine the functional impact of miR-30a on lung cancer cell behavior.

Main Methods:

  • Correlation analysis of MEF2D and miR-30a expression in lung cancer tissues.
  • Bioinformatic prediction of miR-30a target sites on MEF2D mRNA.
  • Luciferase reporter assays to validate MEF2D 3'UTR targeting by miR-30a.
  • Western blot analysis to assess MEF2D protein levels.
  • Cell viability, colony formation, and apoptosis assays (FACS) following miR-30a mimic transfection.

Main Results:

  • MEF2D and miR-30a expression were inversely correlated in lung cancer samples.
  • miR-30a directly targeted the 3' untranslated region (UTR) of MEF2D mRNA.
  • Transfection with miR-30a mimics suppressed MEF2D protein expression.
  • miR-30a mimics reduced lung cancer cell growth and colony formation.
  • miR-30a mimics promoted apoptosis in lung cancer cells.

Conclusions:

  • Aberrant expression of miR-30a in lung cancer is associated with increased MEF2D protein levels.
  • miR-30a functions as a tumor suppressor in lung cancer by inhibiting MEF2D.
  • miR-30a inhibits lung cancer cell proliferation and colony formation by inducing apoptosis.
Keywords:
lung cancermicroRNA-30amyocyte enhancer factor 2D

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