Related Experiment Video
Updated: Feb 8, 2026
08:12
Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
Published on: March 14, 2019
6.0K
Next generation sequencing identifies miRNA-based biomarker panel for lupus nephritis
Yu-Jih Su1, I-Chun Lin2, Lin Wang3
1Department of Rheumatology, Allergy and Immunology, Kaohsiung Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Kaohsiung, Taiwan.
Oncotarget
|July 3, 2018
Summary
Researchers identified miR-146a-5p as a promising biomarker for early lupus nephritis (LN) detection. This microRNA could help diagnose LN sooner, preventing kidney damage in systemic lupus erythematosus (SLE) patients.
Area of Science:
- * Nephrology and Immunology
- * Molecular Biology and Biomarker Discovery
Background:
- * Lupus nephritis (LN) presents with mild, non-specific symptoms like foamy urine and edema.
- * Delayed diagnosis of LN can lead to permanent kidney damage in up to 10% of patients.
- * There is a critical need for reliable biomarkers for early LN detection.
Purpose of the Study:
- * To identify a specific and sensitive surrogate biomarker for the early detection of lupus nephritis.
- * To screen for differentially expressed microRNAs in patients with systemic lupus erythematosus (SLE) with and without LN.
Main Methods:
- * Next-generation sequencing (NGS) was used to screen microRNA expression profiles.
- * Real-time quantitative polymerase chain reaction (qPCR) validated microRNA candidates.
- * Analysis incorporated clinical laboratory data and literature review findings.
Main Results:
- * NGS identified 41 microRNAs with significant differential expression.
- * Real-time qPCR confirmed miR-125a-5p, miR-146a-5p, and miR-221-3p as statistically significant.
- * miR-146a-5p showed a significant correlation with clinical biochemistry markers and emerged as a key surrogate biomarker.
Conclusions:
- * miR-146a-5p is a potential intracellular biomarker for early lupus nephritis detection in SLE patients.
- * This biomarker's efficacy is independent of serum albumin levels and urine protein/creatinine ratios.
- * Early detection using miR-146a-5p could facilitate timely treatment and prevent kidney damage.
Related Concept Videos
03:00Next-generation Sequencing
98.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.7K
01:18Wood Panel Products
400
Wood panel products are essential materials used in construction for applications such as flooring, siding, and roofing, typically available in standard dimensions of 4 feet by 8 feet, with thicknesses varying from one-quarter of an inch to one and one-eighth inches. Among the most common types of wood panels is plywood, which is produced by gluing multiple layers of thin wood veneers under pressure. The grain of the outer veneers runs lengthwise, while the grains of the interior layers run...
400
02:02Cis-regulatory Sequences
11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
01:29Sequences
280
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
280
01:57Sanger Sequencing
774.8K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.8K
01:30Arithmetic Sequences
240
An arithmetic sequence is a structured arrangement of numbers where each term is derived by adding a constant value, known as the common difference, to the previous term. This consistent pattern allows for the efficient computation of any term within the sequence as well as the cumulative sum of multiple terms. The formula for finding the nth term of an arithmetic sequence is:Here, aₙ represents the nth term of the sequence, a is the first term, d is the common difference, and n is the...
240