Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
T Cell Types and Functions01:24

T Cell Types and Functions

2.5K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
2.5K
Multi-input and Multi-variable systems01:22

Multi-input and Multi-variable systems

426
Cruise control systems in cars are designed as multi-input systems to maintain a driver's desired speed while compensating for external disturbances such as changes in terrain. The block diagram for a cruise control system typically includes two main inputs: the desired speed set by the driver and any external disturbances, such as the incline of the road. By adjusting the engine throttle, the system maintains the vehicle's speed as close to the desired value as possible.
In the absence of...
426
Connective Tissue Cell Types01:22

Connective Tissue Cell Types

4.2K
Connective tissue develops from the mesoderm of a developing embryo and consists of cells, fibers, and ground substance: a gel-like material containing large complexes of carbohydrates and proteins. Connective tissue was first identified as a separate tissue family in the 18th century, and Johannes Peter Muller coined the term connective tissue.
Fat cells (adipocytes), smooth muscle cells (myoblasts), and bone cells (osteoblasts) are some connective tissue cell types. Some immune system cells...
4.2K
Types of RNA01:23

Types of RNA

72.9K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biological activity analysis of baicalin nanodrugs: Nanosizing enhances antiviral and anti-inflammatory effects in the treatment of viral pneumonia.

Journal of pharmaceutical analysis·2025
Same author

Mitochondrial Calcium Uniporter Links Acetyl-CoA Metabolism and H3K27 Acetylation to Maintain Glioblastoma Stem Cells.

Cancer research·2025
Same author

Multiple ctDNA- based biomarkers predict benefit from selective RET Inhibition in non-small cell lung cancer patients: exploratory analysis of a prospective study.

Biomarker research·2025
Same author

Cascade Energy Transformation in Hybrid Nanosensitizer Enables Ultrasound-Activated Luminescence Imaging and Enhanced Sonodynamic Therapy.

Journal of the American Chemical Society·2025
Same author

Effectiveness of virtual, group cough modulation therapy for chronic refractory cough.

ERJ open research·2025
Same author

Synthesis of PPy-BiVO<sub>4</sub>-Cu<sup>2+</sup> heterojunction and its visible-light-driven photocatalytic degradation of 2,4-dichlorophenol.

Environmental research·2025

Related Experiment Video

Updated: Feb 8, 2026

TChIP-Seq: Cell-Type-Specific Epigenome Profiling
07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

8.4K

Cell-type-specific brain methylomes profiled via ultralow-input microfluidics.

Sai Ma1, Mario de la Fuente Revenga2, Zhixiong Sun3

  • 1Department of Biomedical Engineering and Mechanics, Virginia Tech, Blacksburg, VA 24061, USA.

Nature Biomedical Engineering
|July 3, 2018
PubMed
Summary
This summary is machine-generated.

Microfluidic diffusion-based reduced representative bisulfite sequencing (MID-RRBS) enables high-quality methylomic profiling from minimal DNA. This technique allows for cell-type-specific DNA methylation analysis, even in drug-treated samples.

More Related Videos

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

8.3K
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
08:37

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

Published on: May 28, 2015

17.5K

Related Experiment Videos

Last Updated: Feb 8, 2026

TChIP-Seq: Cell-Type-Specific Epigenome Profiling
07:28

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

Published on: January 23, 2019

8.4K
Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

8.3K
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
08:37

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

Published on: May 28, 2015

17.5K

Area of Science:

  • Epigenetics
  • Genomics
  • Neuroscience

Background:

  • Traditional methylomic analyses require large DNA quantities, limiting studies with scarce samples.
  • Cell-type-specific DNA methylation is crucial for understanding brain function and disease.

Purpose of the Study:

  • To introduce microfluidic diffusion-based reduced representative bisulfite sequencing (MID-RRBS) for low-input DNA methylomic profiling.
  • To establish cell-type-specific methylomes from mouse brain fractions.
  • To evaluate the utility of MID-RRBS for drug-induced methylome changes.

Main Methods:

  • Utilized a microfluidic device for efficient bisulfite conversion and high DNA recovery.
  • Performed genome-wide DNA methylation analysis on NeuN+ (neuronal) and NeuN- (glial) fractions from mouse brains.
  • Applied MID-RRBS to analyze methylomic landscapes in drug-treated mouse models.

Main Results:

  • Achieved high-quality methylomic profiling using nanogram-to-single-cell quantities of DNA.
  • Identified distinct genome-wide methylation patterns between neuronal and glial cell populations.
  • Detected significant methylomic variations in neuronal cells following antipsychotic drug treatment.

Conclusions:

  • MID-RRBS is a powerful tool for methylomic profiling of scarce cell populations.
  • The technology facilitates cell-type-specific epigenomic studies in neuroscience.
  • MID-RRBS is applicable for drug profiling and investigating drug-methylome interactions.