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Updated: Feb 8, 2026

Pretreatment of Lignocellulosic Biomass with Low-cost Ionic Liquids
Published on: August 10, 2016
Laccase-mediator system produced by Trametes hirsuta Bm-2 on lignocellulosic substrate improves dye decolorization
Wendy Ancona-Escalante1, Raul Tapia-Tussell2, Luis Pool-Yam1
11Depto. Ingeniería Química y Bioquímica, Instituto Tecnológico de Mérida, CP. 97118 Mérida, Yucatán Mexico.
Abstract:
Lignin is a source for obtaining natural phenols with high commercial value that can act as redox mediators enhancing effects in dye decolorization. In this study Trametes hirsuta Bm-2 was grown on wheat bran to produce laccases and phenol extracts (PE). Ultrafiltered phenols obtained at different times were evaluated in their potential as redox mediators of laccase activity and indigo carmin decolorization. Laccase activity (L) on ABTS increased up to 12.4 times with L/PE72 compared with laccase alone and L/PE48 showed the highest level of dye decolorization (97%) compared with laccase (12%). The chromatographic analysis by HPLC showed variation in the profile and concentration of phenols at different times of culture. Stability of the laccase mediator system (LMs) in dye decolorization was maintained over 3 months. Our results suggest the use of natural mediators as a strategy for improving efficiency in dye biodegradation by laccase-producing fungi.
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