Related Experiment Video
Updated: Feb 8, 2026

Orthotopic Mouse Model of Colorectal Cancer
Published on: December 4, 2007
Investigation of GHSR and GHRL methylation in colorectal cancer
Fabio Coppedè1, Andrea Stoccoro1, Alessandro Lazzarotti1
1Department of Translational Research & New Technologies in Medicine & Surgery, Medical Genetics Laboratory, University of Pisa, Pisa, Italy.
Aim:
To investigate GHSR and GHRL methylation in 73 pairs of colorectal cancer (CRC) tissues and healthy adjacent mucosa.
Methods:
Methylation was assessed with methylation-sensitive high-resolution melting.
Results:
GHSR was significantly hypermethylated in CRC tissues than in healthy mucosa (p < 1 × 10-5), but no significant changes of GHRL methylation were observed. GHSR hypermethylation was already detectable at the adenoma stage and maintained in later stages independently of age, gender, anatomical location, histological grading, MLH1 deficiency, as well as of major polymorphisms in folate-pathway genes, yielding an area under the curve of 0.824 for discriminating cancers from respective non-neoplastic mucosa specimens.
Conclusion:
GHSR hypermethylation occurs early in CRC, but is not paralleled by significant changes of GHRL methylation.
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