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Updated: Feb 8, 2026

Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Published on: September 20, 2016
[Fast Start-up and Performance of the CANON Process Based on a SBAF Systemand Evolution Properties of Microorganisms]
Xiu Yue1, Zhu-Han Liu1, Guang-Ping Yu1
1Shenyang Institute of Automation in Guangzhou, Chinese Academy of Sciences, Guangzhou 511458, China.
Abstract:
The CANON process has the disadvantages of long start-up periods and unstable operation. In the SBAF system, under strictly controlled conditions of dissolved oxygen (DO) and temperature, a CANON process is started up in 51 days and is operated stably for 278 days using a new method of independent research and development. The results show that the maximum and average ARR are 98.9% and 95.1%, respectively. The maximum and average TNR are 85.9% and 75.1%, respectively. Furthermore, a small quantity of nitratenitrogen exists in this system. The microbial structure features of the sludge are analyzed by 16S rDNA macro high-throughput genome sequencing. It is verified that Proteobacteria is the main microorganism in the AOB, and Planctomycete Candidatus Brocadia is the main microorganism in the AnAOB. Both of them collaborate on total nitrogen removal in the CANON process.
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