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Updated: Feb 8, 2026

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
Published on: October 15, 2015
[Biological Pre-treatment of Surplus Sludge Using the Protease-secreting Bacteria]
Pin-Jing He1,2, Ying Wang1, Jie Hu1
1Institute of Waste Treatment and Reclamation, Tongji University, Shanghai 200092, China.
Abstract:
The performances of biological pre-treatment were compared at different inoculation ratios of Bacillus licheniformis (0.17%, 0.66%, 1.16% and 1.65%, TS/TS), in order to evaluate the influence on sludge liquefaction and its dewaterability. The analysis showed that biological pre-treatment by inoculating Bacillus licheniformis accelerated the dissolution of intracellular materials and increased the degradation of protein, but the sludge dewaterability deteriorated. When the inoculation ratio of Bacillus licheniformis was 1.16%, the cumulative effect of dissolved organic carbon reached its maximum. Keeping increasing the inoculation ratio would not result in any improvement. At an inoculation ratio of 1.16%, the quantity of protein per volatile solid reached the minimum, which was 72% of the initial value, after 129 hours of digestion, which reflected the maximal degradation rate of protein. But at same time, the value of CST of sludge doubled, which indicated the deterioration of sludge dewaterability.
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