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Updated: Feb 8, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
[Effect of Temperature on PAO Activity and Substrate Competition]
Ling Zhang1, Dang-Cong Peng1, Die Chang1
1School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Abstract:
To investigate the effect of temperature on the activity of high-temperature phosphate accumulating organisms (PAOHT) and their competitive ability for the substrate, the sludge from an enhanced biological phosphorus removal (EBPR) reactor stably operated at the high temperature of 30℃ was used. The results showed that PAOHT activity and their competitive ability for the substrate increased with temperature increasing from 15℃ to 30℃. When the temperature was as higher as 30℃, the rates of anaerobic phosphorus release, aerobic phosphorus uptake and acetate uptake of PAOHT were 239.46 mg·(g·h)-1, 79.90 mg·(g·h)-1, 357.47 mg·(g·h)-1, respectively. The corresponding value of ΔP/ΔHAc was 0.628, which was considerably higher than that of PAO reported in literatures. A simplified Arrhenius equation was used to describe the effect of temperature on the rates of anaerobic phosphorus release, aerobic phosphorus uptake and acetate uptake, and the temperature coefficients were 1.08, 1.07 and 1.05, respectively.
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