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[Nitrifying Bacteria Culture in Entrapment Immobilization]
1Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Huan Jing Ke Xue= Huanjing Kexue
|July 3, 2018
Summary
Industrialized nitrifying bacteria cultivation using enrichment and immobilization techniques significantly boosted ammonia oxidation rates. This method enhanced Nitrosomonas populations and achieved efficient recovery of biologically active fillers for wastewater treatment.
Area of Science:
- Environmental Microbiology
- Biotechnology
- Wastewater Treatment Engineering
Background:
- Industrial application of nitrifying bacteria requires efficient cultivation and immobilization methods.
- Activated sludge from sewage treatment plants is a potential source for nitrifying bacteria.
- Controlling key environmental factors is crucial for optimizing nitrifying bacteria growth.
Purpose of the Study:
- To develop an industrialized method for nitrifying bacteria enrichment cultivation.
- To achieve large-scale application of entrapment immobilization for nitrifying bacteria.
- To enhance ammonia oxidation rates and recover biologically active immobilized nitrifying bacteria.
Main Methods:
- Progressive substrate concentration increase in an industrialized tank.
- Control of free ammonia (FA) and free nitrous acid (FNA) concentrations.
- High-throughput sequencing for bacterial community analysis.
- Polyvinyl alcohol (PVA) embedding for immobilization.
- Continuous-flow approach for recovery phase.
Main Results:
- Ammonia oxidation rate reached 118 mg·(L·h)-1.
- Nitrosomonas content increased from 0.53% to 10.27% after enrichment.
- Bacterial diversity decreased significantly post-enrichment.
- Nitrification loading rate of embedded fillers reached 62 mg·(L·h)-1.
- Efficient recovery of biologically active immobilized fillers was achieved.
Conclusions:
- Enrichment cultivation effectively increases Nitrosomonas populations and ammonia oxidation rates.
- PVA entrapment immobilization is a viable method for large-scale application.
- The developed method allows for efficient recovery of immobilized nitrifying bacteria for wastewater treatment.
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