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Long non-coding RNA KCNQ1OT1 modulates oxaliplatin resistance in hepatocellular carcinoma through miR-7-5p/ ABCC1

Huiqin Hu1, Li Yang1, Lijun Li2

  • 1Department of Chinese Medicine, Affiliated Longhua Central Hospital, Guangdong Medical University, Shenzhen, 518110, Guangdong, China.

Biochemical and Biophysical Research Communications
|July 4, 2018
PubMed

Insights

Long non-coding RNA KCNQ1OT1 promotes oxaliplatin resistance in hepatocellular carcinoma by regulating the miR-7-5p/ABCC1 axis. Targeting this pathway offers a potential new treatment strategy for liver cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) play roles in cancer chemoresistance.
  • The specific mechanisms of lncRNAs in hepatocellular carcinoma (HCC) chemoresistance remain unclear.

Purpose of the Study:

  • To investigate the function and mechanism of KCNQ1OT1 in oxaliplatin resistance in HCC.
  • To explore the KCNQ1OT1/miR-7-5p/ABCC1 regulatory axis in HCC chemoresistance.

Main Methods:

  • Quantitative real-time PCR to detect KCNQ1OT1 expression.
  • Cell proliferation, migration, and invasion assays.
  • Bioinformatics analysis and dual-luciferase reporter assays to confirm target interactions.

Main Results:

  • KCNQ1OT1 was significantly upregulated in oxaliplatin-resistant HCC cells (HepG2, Huh7).
  • KCNQ1OT1 knockdown inhibited proliferation, migration, and invasion, and reduced drug-resistant gene expression (MRP5, MDR1, LRP1).
  • KCNQ1OT1 sponges miR-7-5p, regulating ABCC1 expression via the miR-7-5p/ABCC1 axis.

Conclusions:

  • KCNQ1OT1 promotes oxaliplatin resistance in HCC through the miR-7-5p/ABCC1 pathway.
  • This axis represents a potential therapeutic target for HCC treatment.
Keywords:
ABCC1Hepatocellular carcinomaKCNQ1OT1Oxaliplatin resistantmiR-7-5p

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