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CD8+ T-Cell Density Imaging with 64Cu-Labeled Cys-Diabody Informs Immunotherapy Protocols
Jai Woong Seo1, Richard Tavaré2, Lisa M Mahakian1
1Department of Biomedical Engineering, University of California, Davis, Davis, California.
Summary
Positron emission tomography (PET) with a novel 64Cu-labeled anti-CD8 antibody fragment can noninvasively track CD8+ T cells in normal tissues and tumors, aiding immunotherapy response assessment.
Area of Science:
- Immunology
- Nuclear Medicine
- Oncology
Background:
- Noninvasive imaging of CD8+ T cells is crucial for evaluating immunotherapy efficacy.
- Current methods lack the sensitivity to detect subtle changes in T-cell distribution.
Purpose of the Study:
- To develop and validate a 64Cu-labeled anti-CD8 cys-diabody (64Cu-169cDb) for quantitative PET imaging of CD8+ T cells.
- To assess the sensitivity of this technique in normal and tumor-bearing mice.
Main Methods:
- Radiolabeling of anti-CD8 cys-diabody with 64Cu.
- PET/CT imaging and biodistribution studies in wild-type mice.
- Quantification and mapping of tumor-infiltrating CD8+ T cells in mice treated with immunotherapy.
Main Results:
- 64Cu-169cDb PET detected CD8+ T-cell distribution differences in normal mouse tissues.
- PET imaging accurately visualized and quantified changes in tumor-infiltrating CD8+ T cells in response to immunotherapy.
- Reduced probe circulation time indicated off-target effects from immunotherapy.
Conclusions:
- 64Cu-169cDb enables spatial mapping of CD8+ T cells in organs and tumors.
- ImmunoPET imaging effectively detected therapy-induced changes in tumor-infiltrating CD8+ T cells.
- This method shows promise for preclinical evaluation of immunotherapy response.
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