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Identification of Metal Oxide Nanoparticles in Histological Samples by Enhanced Darkfield Microscopy and Hyperspectral Mapping

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Ultraviolet Hyperspectral Interferometric Microscopy.

Ashkan Ojaghi¹, Meredith E Fay^1,2, Wilbur A Lam^1,2

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, USA.

Scientific Reports

|July 4, 2018

Summary

This summary is machine-generated.

Ultraviolet hyperspectral interferometric microscopy enables label-free molecular imaging of live cells. This new technique provides quantitative spectral analysis with subcellular resolution for biomolecules.

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Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Hyperspectral Imaging as a Tool to Study Optical Anisotropy in Lanthanide-Based Molecular Single Crystals

Published on: April 14, 2020

Area of Science:

Biophotonics and Imaging
Spectroscopy
Cell Biology

Background:

Ultraviolet (UV) spectroscopy is valuable for biochemical analysis.
UV spectroscopy's use in molecular imaging and microscopy is limited.
Challenges exist in applying UV spectroscopy to molecular imaging.

Purpose of the Study:

Introduce ultraviolet hyperspectral interferometric (UHI) microscopy.
Overcome limitations of UV spectroscopy in molecular imaging.
Enable label-free molecular imaging of live cells.

Main Methods:

Utilize coherent detection of optical fields.
Implement UHI microscopy.
Perform quantitative spectral analysis.

Main Results:

Achieved subcellular spatial resolution.
Demonstrated sensitivity to nanometer-scaled structures.
Enabled label-free molecular imaging of endogenous biomolecules in live cells.

Conclusions:

UHI microscopy overcomes challenges in UV molecular imaging.
UHI microscopy allows quantitative spectral analysis of biomolecules.
This method is suitable for label-free imaging of live cells with high resolution.