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Updated: Feb 8, 2026

Engineering Cell-permeable Protein
Published on: December 28, 2009
StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins
Conor L Jacobs1,2,3, Ryan K Badiee2,3,4,5, Michael Z Lin6,7,8
1Graduate Program in Biological Sciences, Stanford University, Stanford, CA, USA.
Abstract:
Robust approaches for chemogenetic control of protein function would have many biological applications. We developed stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease. StaPLs undergo autoproteolysis to cleave proteins by default, whereas protease inhibitors prevent cleavage and preserve protein function. We created StaPLs responsive to different clinically approved drugs to bidirectionally control transcription with zinc-finger-based effectors, and used StaPLs to create single-chain, drug-stabilizable variants of CRISPR-Cas9 and caspase-9.
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