Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Genetic Engineering?00:49

What is Genetic Engineering?

80.3K
Overview
80.3K
Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein01:20

Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein

922
Antiepileptic drugs, such as levetiracetam (Keppra) and brivaracetam (Briviact), have emerged as crucial tools in managing epilepsy. These medications exert their therapeutic effects by targeting the synaptic vesicle protein SV2A, a transmembrane glycoprotein primarily found in the brain.
SV2A is a transmembrane glycoprotein located predominantly in the brain, modulating the release of neurotransmitters for neuronal communication. Both levetiracetam and brivaracetam exhibit a high affinity for...
922
Encoding01:19

Encoding

867
Information enters the brain through encoding, which is the input of information into the memory system. Once sensory information is received from the environment, the brain labels or codes it. The information is then organized with similar information and connected to existing concepts. Encoding occurs through automatic processing and effortful processing.
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
867
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.3K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.3K
Factors Affecting Protein-Drug Binding: Drug-Related Factors01:18

Factors Affecting Protein-Drug Binding: Drug-Related Factors

487
Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In...
487
Factors Affecting Protein-Drug Binding: Drug Interactions01:23

Factors Affecting Protein-Drug Binding: Drug Interactions

607
Drug interactions are a critical aspect of pharmacology and can occur when two or more drugs compete for the same binding site. This competition can result in one drug displacing another, altering the effect of the displaced drug. Drug interactions are complex processes that rely heavily on how much of the displacer drug is present and how strongly it can bind to the same sites as the displaced drug.
Displacement interactions can have varying outcomes, ranging from toxicity to virtually...
607

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: Rewiring oncogenic signalling to precision ablation of metastatic cancer.

Nature biomedical engineering·2026
Same author

Retrospective cohort analysis of influence of mode of injury on reconstructive outcomes following lower extremity trauma.

Journal of clinical orthopaedics and trauma·2026
Same author

Rewiring oncogenic signalling to precision ablation of metastatic cancer.

Nature biomedical engineering·2026
Same author

Improving positively tuned voltage indicators for faster kinetics and higher contrast.

bioRxiv : the preprint server for biology·2026
Same author

Fast analysis and engineering of protein function by microbe-independent deep assembly and screening.

Molecular systems biology·2026
Same author

Primary Palatoplasty.

Oral and maxillofacial surgery clinics of North America·2026

Related Experiment Video

Updated: Feb 8, 2026

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K

StaPLs: versatile genetically encoded modules for engineering drug-inducible proteins.

Conor L Jacobs1,2,3, Ryan K Badiee2,3,4,5, Michael Z Lin6,7,8

  • 1Graduate Program in Biological Sciences, Stanford University, Stanford, CA, USA.

Nature Methods
|July 4, 2018
PubMed
Summary

Researchers developed stabilizable polypeptide linkages (StaPLs) that use protease inhibitors to control protein function. This chemogenetic tool enables precise regulation of biological processes, including gene transcription and protein activity.

More Related Videos

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
08:20

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

8.3K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.8K

Related Experiment Videos

Last Updated: Feb 8, 2026

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K
Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons
08:20

Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

Published on: March 28, 2016

8.3K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.8K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetic Engineering

Background:

  • Chemogenetic control of protein function is crucial for biological research and therapeutic applications.
  • Existing methods for controlling protein activity often lack precision or require specialized reagents.
  • Developing robust systems for inducible protein function is a significant challenge in synthetic biology.

Purpose of the Study:

  • To develop a novel chemogenetic system for robust control of protein function.
  • To engineer stabilizable polypeptide linkages (StaPLs) responsive to clinically approved drugs.
  • To demonstrate the utility of StaPLs in controlling gene transcription and protein activity, including CRISPR-Cas9 and caspase-9.

Main Methods:

  • Designed and constructed stabilizable polypeptide linkages (StaPLs) based on hepatitis C virus protease.
  • Engineered StaPLs to be responsive to various clinically approved drugs.
  • Integrated StaPLs with zinc-finger-based transcription factors for inducible gene expression.
  • Developed single-chain, drug-stabilizable variants of CRISPR-Cas9 and caspase-9 using StaPL technology.

Main Results:

  • StaPLs function by undergoing default autoproteolysis, which is inhibited by protease inhibitors, thereby preserving protein function.
  • Clinically approved drugs were successfully used to modulate StaPL activity, enabling bidirectional control of transcription.
  • Drug-stabilizable single-chain CRISPR-Cas9 and caspase-9 variants were successfully created, demonstrating the system's versatility.
  • The developed chemogenetic system offers precise temporal and chemical control over protein function.

Conclusions:

  • Stabilizable polypeptide linkages (StaPLs) provide a robust and versatile platform for chemogenetic control of protein function.
  • This technology enables precise, drug-inducible regulation of gene expression and protein activity.
  • StaPLs have broad applicability in synthetic biology, molecular biology, and potentially in therapeutic strategies requiring conditional protein activity.