Bilirubin binding in jaundiced newborns: from bench to bedside?

Bilirubin-induced neurologic dysfunction (BIND) is a spectrum of preventable neurological sequelae in jaundiced newborns. Current total plasma bilirubin (B_T) concentration thresholds for phototherapy and/or exchange transfusion poorly predict BIND.

The unbound (free) bilirubin (B_f) measured at these B_T thresholds provides additional information about the risk for BIND. B_f can be readily adapted to clinical use by determining B_f population parameters at current B_T thresholds. These parameters can be established using a plasma bilirubin binding panel (BBP) consisting of B_T, B_f, and two empiric constants, the maximum B_T (B_Tmax) and the corresponding equilibrium association bilirubin constant (K).

B_Tmax and K provide the variables needed to accurately estimate B_f at B_T < B_Tmax to obtain B_f at threshold B_T in patient samples. Once B_f population parameters are known, the BBP in a newborn can be used to identify poor bilirubin binding (higher B_f at the threshold B_T compared with the population) and increased risk of BIND.

The BBP can also be used in jaundice screening to better identify the actual B_T at which intervention would be prudent. The BBP is used with current B_T thresholds to better identify the risk of BIND and whether and when to intervene.