Related Experiment Video
Updated: Jul 15, 2026
13:47
Chromatin Immunoprecipitation (ChIP) using Drosophila tissue
Published on: March 23, 2012
The nucleotide sequence and the tissue-specific expression of Drosophila c-src
Cell
|October 1, 1985
Summary
The Drosophila c-src gene encodes a protein similar to chicken c-src. Its expression is highest in embryos and pupae, particularly in neural tissues and smooth muscle during development.
Area of Science:
- Developmental biology
- Molecular genetics
- Oncogene research
Background:
- The vertebrate proto-oncogene c-src plays a role in cell growth and differentiation.
- Understanding the function of c-src homologs in other species can provide insights into conserved biological processes.
Purpose of the Study:
- To investigate the coding capability and expression patterns of the Drosophila homolog of the vertebrate proto-oncogene c-src.
- To determine the potential role of Drosophila c-src in development.
Main Methods:
- Sequence analysis of a cDNA clone for Drosophila c-src.
- Analysis of transcription of the Drosophila c-src locus into three distinct mRNAs.
- In situ hybridization to examine the spatial and temporal expression of c-src RNA during development.
Main Results:
- Drosophila c-src encodes a 62 kd protein highly similar to chicken c-src.
- The gene is transcribed into three independently regulated mRNAs.
- Drosophila c-src RNA is abundant in embryos and pupae, but scarce in larvae and adults.
- Expression is concentrated in neural tissues (brain, ventral nerve cord, eye-antennal discs) and smooth muscle after 8 hours of development.
Conclusions:
- Drosophila c-src may not function as a mitotic signal.
- c-src likely plays a crucial role in the development of neural tissues and smooth muscle in Drosophila.
Related Concept Videos
02:02Cis-regulatory Sequences
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...