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Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Coronary Circulation01:21

Coronary Circulation

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The heart, an organ critical to survival, gets nourishment not from the blood it pumps but from a separate circulation system known as coronary circulation. This is the shortest circulation in the body and is responsible for supplying the heart with the nutrients it needs to function effectively.
Coronary circulation begins at the base of the aorta, where two main arteries arise—the left and right coronary arteries. These arteries encircle the heart in the coronary sulcus and supply the...
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Fetal Circulation01:14

Fetal Circulation

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Fetal circulation is a unique system that facilitates the exchange of gases, nutrients, and waste products between the developing fetus and the mother. This intricate process takes place through a special organ called the placenta.
Two umbilical arteries transport blood from the fetus to the placenta. At the placenta, the blood absorbs oxygen and nutrients while simultaneously eliminating waste products. This oxygen-enriched and nutrient-rich blood then returns to the fetus through one...
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Overview of Pulmonary Circulation01:19

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The pulmonary circulation is a vital system in our body that acts as a bridge between the respiratory and cardiovascular systems. It serves as a transport network for deoxygenated blood from the heart to the lungs and then returns oxygen-rich blood back to the heart.
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Overview of Systemic and Pulmonary Circulation01:15

Overview of Systemic and Pulmonary Circulation

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The systemic and pulmonary circuits are crucial components of the circulatory system, working together to transport blood between the heart, lungs, and the rest of the body. The process begins with pulmonary circulation, where deoxygenated blood is pumped from the right ventricle to the lungs via the pulmonary trunk and arteries. Upon reaching the lungs, the blood becomes oxygenated and returns to the heart, specifically to the left atrium, via the pulmonary veins.
The oxygenated blood is sent...
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Separation of Sister Chromatids02:17

Separation of Sister Chromatids

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At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
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Related Experiment Video

Updated: Feb 8, 2026

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology
09:45

Microfluidics-based High-throughput Circulating Tumor Cell Sorting and Single-cell Sequencing Technology

Published on: November 14, 2025

663

Circulating Tumor Cell Phenotyping via High-Throughput Acoustic Separation.

Mengxi Wu1, Po-Hsun Huang1, Rui Zhang2

  • 1Department of Mechanical Engineering and Material Science, Duke University, Durham, NC, 27708, USA.

Small (Weinheim an Der Bergstrasse, Germany)
|July 4, 2018
PubMed
Summary

A new platform uses acoustics and microfluidics to isolate circulating tumor cells (CTCs) from blood. This high-throughput method preserves cell integrity for improved cancer diagnostics and prognostics.

Keywords:
acoustofluidicscancer phenotypingcirculating tumor cellshigh-throughput separationmicrofluidics

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Cell Biology

Background:

  • Circulating tumor cells (CTCs) are crucial for cancer diagnostics and prognostics.
  • Current methods for CTC isolation face challenges in throughput and preserving cell integrity.
  • Advanced platforms are needed to fully exploit CTC information for cancer treatment.

Purpose of the Study:

  • To develop a high-throughput platform for isolating rare CTCs from peripheral blood.
  • To preserve the structural, biological, and functional integrity of isolated CTCs.
  • To enable detailed analysis of CTCs for diagnostic and prognostic biomarker discovery.

Main Methods:

  • Integration of acoustics and microfluidics for cell separation.
  • High-throughput isolation of cancer cells from leukocytes at 7.5 mL/h.
  • Acoustic separation to maintain cell proliferation and integrity.

Main Results:

  • Achieved a CTC recovery rate of at least 86%.
  • Enabled statistical analysis of CTC size distribution and phenotypic heterogeneity.
  • Visualized CTCs with loss of prostate-specific membrane antigen expression.
  • Successfully isolated CTC clusters.

Conclusions:

  • The developed platform effectively isolates rare CTCs with high recovery and integrity.
  • This technology facilitates comprehensive CTC analysis for cancer patient stratification.
  • It offers a promising tool for advancing CTC-based cancer diagnostics and prognostics.