Related Experiment Video
Updated: Aug 16, 2026

Isolation and Culture of Endothelial Cells from the Embryonic Forebrain
Published on: January 23, 2014
Polarized plasma membrane domains in cultured endothelial cells
Abstract:
To determine whether distinct plasma membrane domains exist in endothelial cells, we infected monolayer cultures of macro- and microvascular endothelial cells with enveloped RNA viruses known to bud selectively from either the apical or basal surface in polarized epithelial cells. We found that vesicular stomatitis (VSV) and Sendai virus emerge asymmetrically from cultured endothelial cells. This provides direct evidence for the existence of polarized plasma membrane domains in vascular endothelial cells.
Related Concept Videos
Asymmetric Lipid Bilayer
Protein Diffusion in the Membrane
Membrane Domains
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the anterior...
Enlargement of the Plasma Membrane
Phosphoinositides and PIPs
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Mechanisms of Membrane Domain Formation
Another mechanism for membrane domain formation involves membrane proteins interacting with cytoskeletal...

