Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.9K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
What is Gene Expression?01:42

What is Gene Expression?

196.9K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
What is Gene Expression?01:36

What is Gene Expression?

11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

6.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic modification of H2AX renders mesenchymal stromal cell-derived dopamine neurons more resistant to DNA damage and subsequent apoptosis.

Cytotherapy·2016
Same author

Study of LRRK2 variation in tauopathy: Progressive supranuclear palsy and corticobasal degeneration.

Movement disorders : official journal of the Movement Disorder Society·2016
Same author

Analysis of C9orf72 repeat expansions in a large international cohort of dementia with Lewy bodies.

Neurobiology of aging·2016
Same author

TYROBP genetic variants in early-onset Alzheimer's disease.

Neurobiology of aging·2016
Same author

[18F]AV-1451 tau-PET uptake does correlate with quantitatively measured 4R-tau burden in autopsy-confirmed corticobasal degeneration.

Acta neuropathologica·2016
Same author

A genome-wide association study in multiple system atrophy.

Neurology·2016

Related Experiment Video

Updated: Feb 8, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K

TMEM106B haplotypes have distinct gene expression patterns in aged brain.

Yingxue Ren1, Marka van Blitterswijk2, Mariet Allen2

  • 1Department of Health Sciences Research, Mayo Clinic, Jacksonville, FL, USA.

Molecular Neurodegeneration
|July 5, 2018
PubMed
Summary

TMEM106B gene variants influence neurodegenerative disease risk by altering brain gene expression. The risk haplotype (TT) is linked to immune responses and reduced neuronal markers, while the protective haplotype (SS) is associated with synaptic transmission, impacting brain health.

Keywords:
Frontotemporal dementia, Co-expression networks, Differential expressionImmune responseProgranulinSynaptic transmissionTMEM106B

More Related Videos

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
17:38

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

16.1K
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

8.2K

Related Experiment Videos

Last Updated: Feb 8, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue
17:38

Radioactive in situ Hybridization for Detecting Diverse Gene Expression Patterns in Tissue

Published on: April 27, 2012

16.1K
An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions
07:59

An Optogenetic Method to Control and Analyze Gene Expression Patterns in Cell-to-cell Interactions

Published on: March 22, 2018

8.2K

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Single nucleotide polymorphisms (SNPs) in TMEM106B are linked to neurodegenerative disease risk.
  • The TMEM106B risk haplotype (TT) is associated with higher TMEM106B levels and cytotoxicity.
  • The precise mechanism of TMEM106B haplotype involvement in neurodegeneration remains unclear.

Purpose of the Study:

  • To analyze transcriptome signatures associated with TMEM106B risk (TT) and protective (SS) haplotypes.
  • To identify differentially expressed genes and gene networks linked to TMEM106B haplotypes in neurodegenerative disease.
  • To investigate the impact of TMEM106B haplotypes on cell-type composition in the brain.

Main Methods:

  • Utilized RNA sequencing data from temporal cortex (TCX) and cerebellum (CER) of 312 subjects.
  • Employed Analysis of Variance (ANOVA) to find differentially expressed genes.
  • Applied Weighted Gene Co-expression Network Analysis (WGCNA) to identify gene networks.

Main Results:

  • Identified 593 differentially expressed genes in TCX and 7 in CER when comparing TT to SS carriers.
  • Found SS haplotype positively correlated with synaptic transmission networks; TT haplotype with immune response networks.
  • Observed reduced neuronal marker expression in TT carriers compared to SS carriers, particularly in TCX.

Conclusions:

  • TMEM106B haplotypes significantly impact brain gene expression and cell-type composition.
  • Transcriptional differences are conserved across TCX and CER, with striking changes in TCX.
  • Understanding TMEM106B's role is crucial for neurodegenerative disease research.