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Titration of Human Coronaviruses Using an Immunoperoxidase Assay
Published on: April 28, 2008
Canine respiratory coronavirus employs caveolin-1-mediated pathway for internalization to HRT-18G cells
Artur Szczepanski1,2, Katarzyna Owczarek1,2, Aleksandra Milewska1,2
1Virogenetics, Malopolska Centre of Biotechnology, Jagiellonian University, Krakow, Poland.
Abstract:
Canine respiratory coronavirus (CRCoV), identified in 2003, is a member of the Coronaviridae family. The virus is a betacoronavirus and a close relative of human coronavirus OC43 and bovine coronavirus. Here, we examined entry of CRCoV into human rectal tumor cells (HRT-18G cell line) by analyzing co-localization of single virus particles with cellular markers in the presence or absence of chemical inhibitors of pathways potentially involved in virus entry. We also targeted these pathways using siRNA. The results show that the virus hijacks caveolin-dependent endocytosis to enter cells via endocytic internalization.
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