Comparison of Microvessel Density Using Nestin and CD34 in Colorectal Cancer
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Updated: Feb 8, 2026

Orthotopic Mouse Model of Colorectal Cancer
Published on: December 4, 2007
Mariusz Chabowski1,2, Aleksandra Nowak3, Jedrzej Grzegrzolka3
1Division of Surgical Specialties, Department of Clinical Nursing, Faculty of Health Science, 4th Military Teaching Hospital, Wroclaw, Poland; Wroclaw Medical University, Wroclaw, Poland mariusz.chabowski@gmail.com.
Background/Aim:
The aim of the present study was to compare the prognostic value of nestin-positive microvessel density (nestin+ MVD) to the CD34-positive (CD34+) MVD in patients with colorectal cancer.
Materials And Methods:
A total of 125 cases of colorectal cancer were used for the study. Immunohistochemical reactions were performed on paraffin sections using anti-nestin and anti-CD34. The average MVD was determined for both markers and was referenced to patients' clinicopathological data.
Results:
A strong positive correlation was found between the MVDs for both analyzed antigens. Contrary to CD34+ MVD, a positive trend was observed between high nestin+ MVD and lymph node invasion. Moreover, significantly higher nestin+ MVD was noted in patients with more advanced clinical stage than in those with early-stage cancer. No such correlation was found for CD34 antigen. Additionally, a possible trend between the higher nestin+ MVD and shorter event-free survival was noted, whereas no such relation was observed for CD34+ MVD.
Conclusion:
The results suggest that nestin might be a reliable marker for angiogenesis evaluation in patients with colorectal cancer. In contrast to CD34, the expression of nestin in blood vessels seems to be a more sensitive marker of cancer progression.
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