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Isolation of Human Endothelial Cells from Normal Colon and Colorectal Carcinoma - An Improved Protocol
Published on: April 4, 2018
Longan flower proanthocyanidins induce apoptosis in HT-29 colorectal carcinoma spheroids
Yuan-Chiang Chung1, Hua-Che Chiang2, Hsiang Chang3
1Department of Surgery, Cheng Ching Hospital, Chung-Kang Branch, Taichung, Taiwan, China.
Aim Of Study:
Proanthocyanidin-rich longan flower extract (LFP) has been previously shown to inhibit the proliferation and anchorage-independent growth in soft agar of two colorectal carcinoma (CRC) cells in vitro. In this report, we further examined the effects of LFP in a CRC spheroid model.
Materials And Methods:
A liquid-overlay assay employing HT-29 spheroids was used to evaluate the effects of LFP on cancer cell tumorigenesis, viability, and apoptosis. Associated effects on signaling path ways (epidermal growth factor receptor [EGFR], Akt) and apoptotic regulators were measured using Western blot.
Results:
Treatment with LFP up to 200 μg/ml inhibited tumor growth in a dose-dependent manner and induced prominent apoptosis as measured by annexin V staining. Cells treated with LFP showed decreased EGFR and Akt phosphorylation with decreased expression of B-cell lymphoma 2.
Conclusion:
The ability of LFP to induce apoptosis in CRC spheroids warrants further investigation of its composition and identification of tumor-active components.
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