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Genomic DNA sequence for human C-reactive protein.

K J Lei, T Liu, G Zon

The Journal of Biological Chemistry

|October 25, 1985

Summary

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Researchers isolated the human C-reactive protein (CRP) gene, revealing a longer mature protein sequence than previously known. This finding impacts understanding of this key acute phase reactant.

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

C-reactive protein (CRP) is a crucial acute phase reactant in human inflammatory responses.
Previous understanding of CRP's structure was based on amino acid sequencing.

Purpose of the Study:

To isolate and characterize the human C-reactive protein (CRP) gene.
To determine the complete nucleotide sequence and predict the full amino acid sequence of CRP.

Main Methods:

Isolation of human DNA fragments using lambda phage libraries.
Synthetic oligonucleotide probes for gene identification.
Nucleotide sequence analysis of the isolated gene.

Main Results:

The human CRP gene was successfully isolated and sequenced.

Related Experiment Videos

An unusual intron containing poly(A) and poly(GT) regions was identified.

DNA sequence analysis predicts a 206-amino acid mature CRP, differing from the previously reported 187 amino acids.

Identified promoter sequences (TATAAAT, CAAT) and characterized noncoding regions.

Conclusions:

The complete nucleotide sequence provides a more accurate prediction of the mature C-reactive protein structure.
The identified gene structure offers insights into CRP regulation and evolution.
This detailed genetic information is vital for further research into CRP's role in inflammation.