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Updated: Feb 8, 2026

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
Published on: July 27, 2022
High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates
Keith J Mickolajczyk1, William O Hancock2
1Biomedical Engineering Department, Pennsylvania State University, University Park, PA, USA.
Abstract:
This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark-field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced as a specialized means of capturing processive events in a reduced field of view. Finally, step-finding tools for analyzing high frame-rate tracking data are described.
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