Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Yeast mRNA splicing in vitro.

R J Lin, A J Newman, S C Cheng

    The Journal of Biological Chemistry
    |November 25, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Yeast pre-mRNA splicing occurs in vitro using ATP. The excised intron forms a lariat structure, with intermediates mirroring in vivo splicing products.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Adverse reaction surveillance analysis of domestic human papillomavirus vaccines with different production processes].

    Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2025
    Same author

    Heat Waves and Early Birth: Exploring Vulnerability by Individual- and Area-Level Factors.

    GeoHealth·2025
    Same author

    Patient Experience With Absolute Voice Rest Following Phonomicrosurgery: A Qualitative Study.

    The Laryngoscope·2023
    Same author

    [Effect of China Children's Asthma Action Plan on exercise in school-age children with bronchial asthma].

    Zhonghua yi xue za zhi·2022
    Same author

    [Evaluation of the efficacy of Chinese Children's Asthma Action Plan on the long-term management of children with asthma at home].

    Zhonghua yi xue za zhi·2020
    Same author

    [Comparison between the efficiency of two-dimensional shear-wave elastography and four serum fibrosis indicators for the assessment of liver fibrosis in chronic hepatitis B].

    Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2020
    Same journal

    Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

    The Journal of biological chemistry·2026
    Same journal

    Introduction to the Thematic Review Series on Intracellular Protein Degradation. The ubiquitous biology of intracellular protein degradation: a tribute to Alfred L. ("Fred") Goldberg.

    The Journal of biological chemistry·2026
    Same journal

    Correction: Aromatic residue-rich amino-terminal segments of temporin L self-assemble into collagen-mimetic peptides with cell-adhesion properties.

    The Journal of biological chemistry·2026
    Same journal

    YhbO is a DJ-1 family glyoxalase and α-oxoaldehyde hydratase that confers resistance to reactive carbonyl stress (112).

    The Journal of biological chemistry·2026
    Same journal

    ARMH3 acts as a central scaffold at the Golgi/TGN through interactions with Arl5, GBF1, and PI4KB.

    The Journal of biological chemistry·2026
    Same journal

    PAX8 controls proximal tubule epithelial identity and stress response through epigenetic modification of distal regulatory elements.

    The Journal of biological chemistry·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • RNA Splicing
    • Yeast Genetics

    Background:

    • Pre-messenger RNA (pre-mRNA) splicing is a crucial process in gene expression.
    • Understanding the mechanisms of splicing in vitro provides insights into in vivo processes.
    • Yeast is a model organism for studying fundamental cellular processes like splicing.

    Purpose of the Study:

    • To investigate the in vitro splicing of synthetic yeast actin and CYH2 pre-mRNAs.
    • To identify the products and intermediates of the splicing reaction in a soluble whole cell extract.
    • To determine the energy requirements and structural features of the excised intron.

    Main Methods:

    • In vitro splicing assays using synthetic yeast actin and CYH2 pre-mRNAs.
    • Analysis of reaction products using gel electrophoresis and Northern blotting.

    Related Experiment Videos

  • Characterization of the excised intron structure.

    • Main Results:

    • Accurate splicing of pre-mRNAs was achieved in a soluble whole cell yeast extract.
    • Splicing reaction requires Adenosine Triphosphate (ATP).
    • The excised intron is released as a lariat structure, with specific branch point.
    • Identified potential reaction intermediates: free linear exon 1 and an extended intron lariat.

    Conclusions:

    • Yeast pre-mRNA splicing can be reconstituted in vitro, requiring ATP.
    • The in vitro splicing pathway generates lariat introns and intermediates consistent with in vivo observations.
    • This system provides a valuable tool for studying the molecular mechanisms of yeast pre-mRNA splicing.