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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle GUV Membranes
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Timing of ESCRT-III protein recruitment and membrane scission during HIV-1 assembly

Daniel S Johnson1,2, Marina Bleck1, Sanford M Simon1

  • 1Laboratory of Cellular Biophysics, The Rockefeller University, New York, United States.

Elife
|July 5, 2018
PubMed

The Endosomal Sorting Complexes Required for Transport III (ESCRT-III) proteins are critical for cellular membrane scission processes with topologies inverted relative to clathrin-mediated endocytosis. Some viruses appropriate ESCRT-IIIs for their release. By imaging single assembling viral-like particles of HIV-1, we observed that ESCRT-IIIs and the ATPase VPS4 arrive after most of the virion membrane is bent, linger for tens of seconds, and depart ~20 s before scission. These observations suggest that ESCRT-IIIs are recruited by a combination of membrane curvature and the late domains of the HIV-1 Gag protein. ESCRT-IIIs may pull the neck into a narrower form but must leave to allow scission. If scission does not occur within minutes of ESCRT departure, ESCRT-IIIs and VPS4 are recruited again. This mechanistic insight is likely relevant for other ESCRT-dependent scission processes including cell division, endosome tubulation, multivesicular body and nuclear envelope formation, and secretion of exosomes and ectosomes.

Keywords:
ESCRTHIV-1cell biologyhumaninfectious diseasemembrane scissionmicrobiologyviral assembly

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