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Related Experiment Videos

Polymyxin B use does not ensure endotoxin-free solution.

M J Kluger, R Singer, S M Eiger

    Journal of Immunological Methods
    |October 24, 1985
    PubMed
    Summary
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    Polymyxin B, used to remove endotoxin in lab tests, only binds some endotoxin. This finding requires re-evaluation of studies relying on polymyxin B for endotoxin inactivation.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Pharmacology

    Background:

    • Polymyxin B is commonly added to in vitro samples to neutralize endotoxin activity.
    • Endotoxins are lipopolysaccharides from Gram-negative bacteria, known for their pyrogenic properties.

    Purpose of the Study:

    • To evaluate the efficacy of polymyxin B in removing endotoxin activity from in vitro samples.
    • To determine if polymyxin B, when immobilized on a gel support, can completely remove endotoxin.

    Main Methods:

    • Standard rabbit pyrogen test (RPT).
    • Limulus amebocyte lysate (LAL) assay.
    • Immobilization of polymyxin B onto a gel support.

    Main Results:

    • Polymyxin B bound to a gel support demonstrated partial, not complete, binding of endotoxin.

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  • Data suggest that polymyxin B does not eliminate all endotoxin activity under tested conditions.

    • Conclusions:

    • The effectiveness of polymyxin B in completely removing endotoxin activity is questionable.
    • Studies that utilized polymyxin B for endotoxin inactivation need re-evaluation based on these findings.