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Related Concept Videos

Isothermal Processes01:21

Isothermal Processes

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A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes.
An ideal gas can also undergo isothermal expansion or compression.
For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

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Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Appendicitis-II: Diagnostic Studies and Management01:29

Appendicitis-II: Diagnostic Studies and Management

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Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
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Myasthenia Gravis: Diagnostic Tests01:15

Myasthenia Gravis: Diagnostic Tests

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Myasthenia gravis is an autoimmune condition affecting neuromuscular transmission, causing generalized weakness in skeletal muscles. Initial diagnoses rely on patients' signs, symptoms, and medical history. The challenge lies in distinguishing myasthenia from other muscular dystrophies. An important diagnostic feature is the significant improvement of symptoms after administering anticholinesterase inhibitors.
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
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Asthma-IV: Diagnostic and Management01:30

Asthma-IV: Diagnostic and Management

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The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
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Related Experiment Video

Updated: Feb 8, 2026

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
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On chip quadruplex priming amplification for quantitative isothermal diagnostics.

C Pease1, G E Plum2, B Kankia3

  • 1Department of Physics, The Ohio State University, Columbus, OH, 43210, USA.

Biomedical Microdevices
|July 6, 2018
PubMed
Summary
This summary is machine-generated.

Quadruplex Priming Amplification (QPA) is now available on microfluidic chips for point-of-care diagnostics. This integration enables efficient nucleic acid testing with performance comparable to existing methods.

Keywords:
Isothermal amplificationLab on a ChipMicrofluidicsQPAQuadruplex priming amplification

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Area of Science:

  • Biotechnology
  • Medical Diagnostics
  • Molecular Biology

Background:

  • Nucleic acid testing is crucial for medical diagnostics, including monitoring HIV viral load.
  • Quadruplex Priming Amplification (QPA) is an isothermal nucleic acid amplification method ideal for point-of-care (POC) diagnostics due to low power and reagent needs.

Purpose of the Study:

  • To demonstrate a real-time, exponential QPA reaction integrated into a microfluidic chip for the first time.
  • To assess the performance and compatibility of QPA within a microfluidic device for enhanced diagnostics.

Main Methods:

  • Development and implementation of a Quadruplex Priming Amplification (QPA) assay on a microfluidic platform.
  • Real-time monitoring of the exponential amplification reaction within the microfluidic chip.

Main Results:

  • Successful demonstration of real-time, exponential QPA in a microfluidic chip.
  • The QPA reaction within the microfluidic device was not inhibited.
  • Performance levels achieved were comparable to a commercial, non-microfluidic QPA setup.

Conclusions:

  • Microfluidic integration is a viable strategy to optimize QPA assays for POC diagnostics.
  • This approach maintains assay performance while offering automation and reduced reagent costs.
  • The study establishes a foundation for advanced microfluidic nucleic acid testing solutions.