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Living Supramolecular Polymerization of DNA.

Laura A Lanier1, Harry Bermudez1

  • 1Department of Polymer Science and Engineering, University of Massachusetts Amherst, 120 Governors Dr, Amherst, MA, 01003, USA.

Macromolecular Rapid Communications
|July 6, 2018
PubMed
Summary

DNA hybridization chain reaction (HCR) is a living supramolecular polymerization. This method offers controlled molecular weights and narrow dispersity, avoiding cyclization, unlike traditional DNA concatemerization.

Keywords:
concatemersdispersityhybridization chain reactionliving polymerizationself-assembly

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Area of Science:

  • Biochemistry
  • Polymer Chemistry
  • Structural DNA Nanotechnology

Background:

  • Supramolecular polymerization has seen recent synthetic advancements.
  • DNA supramolecular polymerization, specifically concatemerization, is known but yields broad molecular weight distributions and cyclization.
  • Concatemerization is a step-like polymerization process.

Purpose of the Study:

  • To demonstrate that DNA hybridization chain reaction (HCR) is a living polymerization process.
  • To highlight the advantages of HCR over concatemerization for DNA polymerization.
  • To explore new opportunities in DNA nanotechnology and molecular biology.

Main Methods:

  • Investigating the polymerization kinetics of DNA hybridization chain reaction (HCR).
  • Analyzing molecular weight distribution and dispersity of HCR polymers.
  • Comparing HCR polymerization with traditional DNA concatemerization.

Main Results:

  • DNA hybridization chain reaction (HCR) exhibits living polymerization characteristics.
  • HCR allows for continued polymerization upon monomer addition after initial consumption.
  • Molecular weight can be controlled by adjusting the monomer to initiator ratio.
  • HCR produces polymers with narrow dispersity and avoids cyclization.
  • HCR offers a significant advantage over concatemerization, which suffers from broad molecular weight distributions and cyclization.

Conclusions:

  • The living character of DNA HCR polymerization is identified.
  • HCR presents a superior method for DNA supramolecular polymerization compared to concatemerization.
  • This finding opens new avenues for structural DNA nanotechnology and molecular biology applications.