Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose
Luis Felipe Clemente1, María Luisa Hernáez1, Antonio Ramos-Fernández2
1Proteomics Unit, Faculty of Pharmacy , Complutense University of Madrid (UCM) , Plaza Ramón y Cajal s/n , 28040 Madrid , Spain.
Abstract:
Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.
More Related Videos
07:27Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
Published on: August 31, 2022
10:50Author Spotlight: Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats
Published on: April 7, 2023
Related Concept Videos
Mesenchymal Stem Cells
Tissue Renewal without Stem Cells
However, failure of such a system...
Stem Cell Therapy for Tissue Regeneration
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
Adult Stem Cells
ATP Synthase: Structure
ATP Synthase: Mechanism
