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Updated: Feb 8, 2026
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Monitoring and Molecular Characterization of Staphylococcus aureus Isolated from Chronic Wounds
Bruna Maiara Ferreira Barreto Pires1, Fernanda Pessanha de Oliveira, Beatriz Guitton Renaud Baptista de Oliveira
1At the Universidade Federal Fluminense in Niterói, Rio de Janeiro, Brazil, Bruna Maiara Ferreira Barreto Pires, MA, and Fernanda Pessanha de Oliveira, MA, are Doctoral Students; and Beatriz Guitton Renaud Baptista de Oliveira, PhD; Patrícia dos Santos Claro Fuly, PhD; Bernadete Teixeira Ferreira-Carvalho, PhD; Geraldo Renato de Paula, PhD; and Lenise Arneiro Teixeira, PhD, are Professors. Acknowledgments: This work was supported by grants from MCT/CNPq/MEC/Capes (no. 06/2011 Casadinho/Procad), CNPq Universal (no. 466064/2011-2), and FAPERJ (no. E-26/010.002812/2014). The authors have disclosed no other financial relationships related to this article. Submitted August 21, 2017; accepted in revised form December 19, 2017.
Objective:
Researchers analyzed chronic wounds treated with 2% hydrogel to determine whether the presence of methicillin-resistant Staphylococcus aureus (MRSA) is related to the presence of clinical signs of infection.
Methods:
Thirty-five patients were recruited for this descriptive study using a quantitative approach. Staphylococcus aureus was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Antibiotic susceptibility was determined using a disk diffusion test according to Clinical and Laboratory Standards Institute standards. Polymerase chain reaction, pulsed-field gel electrophoresis, and multilocus sequence typing were performed. Statistical analyses were performed using Spearman correlation coefficients for the variables MRSA and clinical signs of infection.
Main Outcome Measures:
The identification of MRSA or methicillin-sensitive S aureus (MSSA), presence or absence of an infection in the wound, and molecular characterization of bacteria were measured.
Main Results:
Of the 35 patients analyzed, 8 (22.9%) were classified as having an infection in their wounds. Spearman ρ indicated a strong positive correlation between the increase in the number of clinical signs of infection and MSSA (P =.84), but only a moderate positive correlation with MRSA (P =.60). The S aureus clonal pattern was unique for each of the major bacteria isolated. Global MRSA sequence-type clones (ST-1 and ST-72) were detected in 2 patients.
Conclusions:
Compared with those colonized by MSSA, chronic wounds colonized by MRSA did not display a strong correlation with the presence of a greater number of clinical signs of infection.
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