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Rearrangements between the operators in the bacteriophage lambda.

F H Stephenson, W P Diehl

    Molecular & General Genetics : MGG
    |January 1, 1985
    PubMed
    Summary
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    Lambda phage operator mutations impact infection efficiency. A specific inversion in the lambda iri mutant restores left operon control, enabling proper infection through altered repressor binding.

    Area of Science:

    • Molecular Biology
    • Bacteriology
    • Genetics

    Background:

    • The left operator mutant lambda v2s exhibits impaired development during infection due to uncontrolled left operon expression.
    • Bacteriophage lambda's regulatory regions, including operators OL and OR, are crucial for controlling gene expression and infection cycles.

    Purpose of the Study:

    • To investigate the genetic basis of a revertant mutant (lambda iri) that overcomes the developmental defect of lambda v2s.
    • To elucidate the mechanism by which the lambda iri mutation restores proper regulation of the left operon.

    Main Methods:

    • Genetic analysis of lambda v2s and its revertant lambda iri.
    • Characterization of operator mutations and sequence rearrangements using molecular techniques.
    • Assessment of phage development and gene expression during infection.

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    Main Results:

    • The lambda iri revertant contains an inversion within the cI region, spanning the lambda operators OL and OR.
    • Formation of this inversion is linked to a translocation of right operator (OR) mutant sequence to the left operator (OL) in the lambda v2s mutant.
    • The inverted OR sequence at the OL position in lambda iri restores functional repression of the left operon by the lambda cro repressor.

    Conclusions:

    • The specific inversion in lambda iri corrects the constitutive left operon expression seen in lambda v2s.
    • This genetic rearrangement highlights the critical role of operator sequences and their positioning in bacteriophage lambda gene regulation and infection viability.