Related Experiment Video
Updated: Feb 8, 2026

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods
Published on: July 17, 2019
A feasible and practical 1H NMR analytical method for the quality control and quantification of bioactive principles
Li-Ying Hsieh1, Hsiu-Hui Chan1, Ping-Chung Kuo1,2
1Department of Chemistry, National Cheng Kung University, Tainan 701, Taiwan.
Abstract:
Lycii Fructus, a solanaceous drug, is widely used as functional foods and in Traditional Chinese Medicine. Samples collected from different regions of China have been found to be not identical in chemical compositions which might affect the biological activities. Although many chromatographic and spectrometric methods have been reported to determine the concentration of betaine and other bioactive amino acids, disturbance resulted from other polar substances with low UV-absorbance and expensive mass facilities reduced the applicability of these techniques. In the present study, the strong cation exchange solid phase extraction procedure incorporated with 1H NMR was successfully developed as a rapid and reliable method that can simultaneously determine betaine, citric acid, threonine, alanine, and proline in various Lycii Fructus. In addition, ERETIC 2 method based on PULCON principle was also applied and compared with conventional method. This feasible and practical method offers a very powerful tool for the quality control of commercial Lycii Fructus from different sources.
Related Concept Videos
Quality Control
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
Development of Analytical Methods
Birth Control Methods
The Uncertainty Principle
Hardy-Weinberg Principle
The Pauli Exclusion Principle

