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Endoplasmic Reticulum Stress Mediated MDRV p10.8 Protein-Induced Cell Cycle Arrest and Apoptosis Through the

Quanxi Wang1,2, Xiaoqin Yuan1,2, Yuan Chen1,2

  • 1College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China.

Frontiers in Microbiology
|July 7, 2018
PubMed

Insights

Muscovy duck reovirus p10.8 protein triggers endoplasmic reticulum (ER) stress, leading to cell cycle arrest and apoptosis in chicken cells via the PERK/eIF2α pathway.

Area of Science:

  • Virology
  • Cell Biology
  • Molecular Biology

Background:

  • Muscovy duck reovirus (MDRV) is a pathogen affecting avian health.
  • Understanding the molecular mechanisms of viral pathogenesis is crucial for developing control strategies.
  • The role of viral proteins in inducing cellular stress responses, like endoplasmic reticulum (ER) stress, is an active area of research.

Purpose of the Study:

  • To investigate the pathogenic mechanism of the MDRV p10.8 protein.
  • To elucidate the role of endoplasmic reticulum (ER) stress in MDRV p10.8 protein-induced cellular dysfunction.
  • To determine the specific cellular pathways involved in p10.8 protein-mediated pathogenesis.

Main Methods:

  • Transfection of chicken embryo fibroblasts (DF1) with p10.8 protein expression vectors.
  • Western blotting to analyze protein phosphorylation and expression levels (PERK, eIF2α, CDK2, CDK4, Cyclin E, CHOP, Caspase-12, Caspase-3).
  • Co-immunoprecipitation (Co-IP) to assess protein interactions (BiP and PERK).
  • Flow cytometry to analyze cell cycle distribution.
  • Gene knockdown experiments (PERK, eIF2α) and treatment with ER stress modulators (activator, inhibitor, Tunicamycin, TUDCA).

Main Results:

  • MDRV p10.8 protein transfection increased phosphorylation of PERK and eIF2α, and dissociation of BiP from PERK.
  • p10.8 protein induced ER stress, confirmed by activator/inhibitor treatments and gene knockdown.
  • p10.8 protein caused G0/G1 cell cycle arrest by downregulating cell cycle proteins (CDK2, CDK4, Cyclin E).
  • p10.8 protein induced apoptosis, evidenced by increased expression of apoptosis-related proteins (CHOP, cleaved-Caspase12, cleaved-Caspase3).
  • ER stress and subsequent apoptosis were mediated through the PERK/eIF2α pathway.

Conclusions:

  • MDRV p10.8 protein induces endoplasmic reticulum (ER) stress in chicken cells.
  • This ER stress leads to cell cycle arrest at the G0/G1 phase.
  • The p10.8 protein also triggers apoptosis via the ER stress response.
  • The PERK/eIF2α signaling pathway is central to both cell cycle arrest and apoptosis induced by MDRV p10.8 protein.
Keywords:
MDRVPERKapoptosiscell cycle arresteIF2αendoplasmic reticulum stress

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