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Colorimetric detection of Cu2+ based on the formation of peptide-copper complexes on silver nanoparticle surfaces
Gajanan Sampatrao Ghodake1, Surendra Krishna Shinde1, Rijuta Ganesh Saratale2
1Department of Biological and Environmental Science, College of Life Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, 10326, Goyang-si, Gyeonggi-do, Republic of Korea.
Abstract:
We developed a colorimetric method for the rapid detection of copper ions (Cu2+) in aqueous solution. The detection of Cu2+ is based on coordination reactions of Cu2+ with casein peptide-functionalized silver nanoparticles (AgNPs), leading to a distinct color change of the solution from yellow to red. The developed method has a good detection limit of about 0.16 µM Cu2+ using 0.05 mL of AgNPs stock solution and a linearity in the range of 0.08-1.44 µM Cu2+ with a correlation coefficient of R2 = 0.973. The developed method is a useful tool for the detection of Cu2+ ions. Furthermore, it can be used for monitoring Cu2+ in water at concentrations below the safe limit for drinking water set by the World Health Organization.
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