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Cloning and Differential Expression Analyses of Cdc42 from Sheep
Yong-Jie Yang1,2, Zeng-Shan Liu1, Shi-Ying Lu1
1Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
Journal of Veterinary Research
|July 7, 2018
Summary
Investigating sheep cell division cycle 42 (OaCdc42) mRNA expression revealed it is not a reliable biomarker for differentiating brucellosis infection from vaccination in sheep.
Area of Science:
- Veterinary immunology
- Molecular diagnostics
- Sheep health
Background:
- Brucellosis diagnosis in sheep is challenging due to difficulties in distinguishing infected from vaccinated animals.
- Development of novel diagnostic biomarkers is crucial for accurate differential diagnosis of brucellosis.
Purpose of the Study:
- To investigate the potential of ovine cell division cycle 42 (OaCdc42) mRNA as a biomarker for differential diagnosis of brucellosis in sheep.
- To analyze the tissue distribution and differential expression of OaCdc42 mRNA in infected and vaccinated sheep.
Main Methods:
- Ovine Cdc42 (OaCdc42) cDNA was cloned using rapid amplification of cDNA ends (RACE).
- Tissue distribution of OaCdc42 mRNA was analyzed.
- Differential expression levels of OaCdc42 mRNA in infected and vaccinated sheep were quantified using RT-qPCR.
Main Results:
- OaCdc42 mRNA was detected in various sheep tissues, with the highest expression in the small intestine.
- OaCdc42 mRNA levels differed significantly between infected/vaccinated sheep and controls at specific time points post-inoculation.
- Expression patterns of OaCdc42 mRNA showed significant differences between vaccinated and infected sheep on days 14, 30, and 60 post-inoculation, but not on day 40.
Conclusions:
- Ovine Cdc42 (OaCdc42) mRNA expression patterns are irregular and do not provide a reliable means to differentiate between brucellosis infection and vaccination in sheep.
- OaCdc42 is not a suitable diagnostic biomarker for the differential diagnosis of brucellosis in sheep.
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