Related Experiment Video
Updated: Feb 8, 2026
06:14
Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
Published on: October 6, 2022
2.3K
Sub-saturating Multiphase Flash Irradiances to Estimate Maximum Fluorescence Yield.
Thomas J Avenson1,2, Aaron J Saathoff3,4
1LI-COR Biosciences, Lincoln, NE, USA. tom.avenson@licor.com.
Methods in Molecular Biology (Clifton, N.J.)
|July 7, 2018
Summary
This study introduces a novel method to accurately estimate maximum chlorophyll fluorescence (Fm') in plants. By analyzing the hyperbolic relationship between fluorescence yield and light intensity, researchers can improve photosynthesis measurements.
Area of Science:
- Plant physiology
- Photosynthesis research
- Chlorophyll fluorescence
Background:
- Leaf-level photosynthesis is studied using gas analysis and chlorophyll fluorometry.
- Maximum fluorescence yield (Fm") is crucial but often underestimated, affecting photosynthesis parameter accuracy.
- Fm" is theoretically approached asymptotically with increasing irradiance.
Purpose of the Study:
- To develop a reliable method for accurately estimating Fm" in light-adapted leaves.
- To address the underestimation issue of Fm" in photosynthesis research.
- To improve the accuracy of photosynthesis measurements derived from Fm".
Main Methods:
- Utilizing pulse-amplitude-modulation chlorophyll fluorometry and infrared gas analysis.
- Employing dynamic multiphase flashes of sub-saturating irradiance (~1 s).
- Generating a reciprocal plot of fluorescence yield against the reciprocal of irradiance for linear regression and extrapolation.
Main Results:
- Demonstrated a hyperbolic relationship between fluorescence yield (ΦF) and irradiance.
- Established a linear, inverse relationship between ΦF and the reciprocal of irradiance (reciprocal plot).
- Successfully extrapolated reciprocal plot data to accurately estimate Fm" at infinite irradiance.
Conclusions:
- The reciprocal plot method provides an accurate estimation of Fm" even with sub-saturating irradiance data.
- This technique overcomes the underestimation problem associated with traditional Fm" measurements.
- Accurate Fm" estimation enhances the reliability of various photosynthesis parameters and light reaction assessments.
Related Concept Videos
01:59Solution Equilibrium and Saturation
22.0K
Imagine adding a small amount of sugar to a glass of water, stirring until all the sugar has dissolved, and then adding a bit more. You can repeat this process until the sugar concentration of the solution reaches its natural limit, a limit determined primarily by the relative strengths of the solute-solute, solute-solvent, and solvent-solvent attractive forces. You can be certain that you have reached this limit because, no matter how long you stir the solution, undissolved sugar remains. The...
22.0K
01:31ATP Yield
79.0K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
79.0K
02:22Reaction Yield
60.0K
The theoretical yield of a reaction is the amount of product estimated to form based on the stoichiometry of the balanced chemical equation. The theoretical yield assumes the complete conversion of the limiting reactant into the desired product. The amount of product that is obtained by performing the reaction is called the actual yield, and it may be less than or (very rarely) equal to the theoretical yield.
60.0K
01:13Maximum Deflection
1.0K
When analyzing beams under unsymmetrical loads, such as a train moving on a bridge, it is crucial to accurately determine the points of maximum stress and deflection. The process involves identifying the maximum deflection of the beam, which may not always occur at its midpoint due to the uneven distribution of the load.
The maximum deflection occurs at a specific point, known as point O, where the tangent to the deflection curve is horizontal. To find point O, the slope of the tangent at any...
The maximum deflection occurs at a specific point, known as point O, where the tangent to the deflection curve is horizontal. To find point O, the slope of the tangent at any...
1.0K
01:16Maximum Power Transfer
934
Numerous practical applications within engineering disciplines, such as telecommunications, necessitate optimizing power delivery to a connected load. This pursuit, however, entails inherent internal losses, which can either equal or exceed the power supplied to the load. The Thevenin equivalent circuit is helpful in finding the maximum power a linear circuit can deliver to a load. It is assumed in this context that the load resistance can be adjusted.
By substituting the entire circuit with...
By substituting the entire circuit with...
934
01:12Maximum Size of Aggregate
564
The maximum size of aggregate is defined as the aperture of the sieve retaining 15 percent or more of the particles present in the aggregate sample. The aggregate's maximum size impacts the concrete's water requirement, workability, and strength. Larger aggregates reduce the surface area needing cement paste coverage, which can lower water needs, thereby allowing a decrease in the water-to-cement ratio when the desired workability and richness of the mix are to be maintained, which can...
564